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190313-Western blots- Lee YK / 1. Buffers 2. western sample Áغñ 3. SDS page 4. Western sample preparation 5. MAM Protein Lysis / 1. Buffers Western blotting¿¡¼ ¹öÆÛ´Â ½ÇÇèÀÇ ¼º°ø¿¡ Áß¿äÇÑ ¿ªÇÒÀ» ÇÑ´Ù. ÀϹÝÀûÀ¸·Î »ç¿ëµÇ´Â ¹öÆÛ´Â »ùÇÃÀ» ÁغñÇÏ°í ´Ü¹éÁúÀ» Àü±â¿µµ¿ÇÏ´Â °úÁ¤¿¡¼ °¢°¢ÀÇ ´Ü°è¿¡¼ ÀûÀýÇÑ È¯°æÀ» Á¦°øÇÑ´Ù. ÀÌ °úÁ¤¿¡¼ »ç¿ëµÇ´Â ´ëÇ¥ÀûÀÎ ¹öÆÛ¿¡¡¦ |
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Western blotting ·¹Æ÷Æ® (1) / 1. Clostridium perfringens¿Í western blotting 2. Western blottingÀÇ Á¤ÀÇ 3. Western blottingÀÇ °úÁ¤ ¹× ¿ø¸® / 1. Clostridium perfringens¿Í western blotting Clostridium perfringens´Â ±×¶÷ ¾ç¼º Çø±â¼º ¼¼±ÕÀ¸·Î, ¿©·¯ °¡Áö µ¶¼Ò¸¦ »ý¼ºÇÏ´Â º´¿ø±ÕÀÌ´Ù. ÁÖ·Î ½ÄÁßµ¶ÀÇ ¿øÀαÕÀ¸·Î ¾Ë·ÁÁ® ÀÖÀ¸¸ç, »óó °¨¿°¿¡¼µµ Áß¿äÇÑ ¿ªÇÒÀ» ÇÑ´Ù. ÀÌ¡¦ |
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Western Blotting ·¹Æ÷Æ® / 1. Background 1-1 Western Blotting 1-2 Sample preparation 1-3 Gel preparation ¹× Electrophoresis 1-4 SDS-PAGE 1-5 Transfer 1-6 Buffer (Sample loading buffer, Running buffer, Transfer buffer) 1-7 Blocking 1-8 Abs reaction 1-9 Detection 2. Purpose and Method 2-1 Purpose 2-2 Method 3. Result - 3-1 Western blotting ban¡¦ |
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SDS PAGE¿Í Western blotting ½ÇÇè report / 1. ½ÇÇè¸ñÀû 2. ÀÌ·Ð 3. ½ÇÇè ½Ã¾à Á¶¼º 4. ½ÇÇè ¼ø¼ 5. °á°ú ¹× Çؼ® / 1. ½ÇÇè¸ñÀû SDS PAGE¿Í Western blotting ½ÇÇèÀÇ ¸ñÀûÀº »ý¹°ÇÐÀû »ùÇÿ¡¼ ´Ü¹éÁúÀÇ ºÐ¸®, Á¤·® ¹× È®ÀÎÀ» ¼öÇàÇÏ´Â °ÍÀÌ´Ù. SDS PAGE´Â sodium dodecyl sulfate polyacrylamide gel electrophoresisÀÇ ¾àÀÚ·Î, ´Ü¹éÁúÀÇ ºÐÀÚ·®¿¡ µû¶ó ºÐ¸®ÇÏ´Â Àü±â¿µµ¿ ±â¡¦ |
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[ÀÇÇÐ,¾àÇÐ] ¿µ¾ç»ýÈÇÐ ½ÇÇè - SDS-PAGE¿¡ ÀÇÇÑ ´Ü¹éÁú ºÐ¸®¿Í Western Blotting / ¿µ¾ç»ýÈÇÐ ½ÇÇè - SDS-PAGE¿¡ ÀÇÇÑ ´Ü¹éÁú ºÐ¸®¿Í Western Blotting ½ÇÇèÁ¦¸ñ SDS-PAGE¿¡ ÀÇÇÑ ´Ü¹éÁú ºÐ¸®¿Í Western Blotting ½ÇÇè¸ñÇ¥ SDS-PAGE·Î ´Ü¹éÁúÀ» ºÐ¸®ÇÑ ÈÄ Àü±â¿µµ¿À» ÅëÇØ ´Ü¹éÁúÀÇ ºÐÀÚ·®À» ÃøÁ¤, ´Ü¹éÁúÇ×üÀÌ¿ë ºÐ¼®À» ÇÒ ¼ö ÀÖ´Ù. ½ÇÇè±â±¸ ¹× ½Ã¾à pipette, ¸¶ÀÌÅ©·Î ÇÇÆê, e.p Æ©¡¦ |
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º» ÀÚ·á´Â western blotting ½ÇÇè ¸®Æ÷Æ®ÀÔ´Ï´Ù. ·¹Æ÷Æ®7 / 1. Introduction 2. Material & Methods 3. Results 4. Discussion 5. Reference / 4. Discussion ¢¡ À§ÀÇ ½ÇÇè°á°ú¸¦ º¸¸é, 1Á¶¿¡¼´Â EpithinÀ» blot Çߴµ¥ ¿ì¸®°¡ ½ÇÇè Àü¿¡ ¿¹»óÇß´ø 92kDa°ú 110kDa ÀÇ µÎ °¡Áö ¹êµå°¡ ³ªÅ¸³ªÁö ¾Ê¾Ò°í 110kDaÀÇ ¹êµå¸¸ÀÌ ³ªÅ¸³ °ÍÀ» º¼ ¼ö ÀÖ¾ú´Ù. EpithinÀÌ transfectionµÇÁö ¾ÊÀº cel¡¦ |
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WESTERN BLOTTINGÀÇ ¿ø¸®, ¹æ¹ý, ¿ëµµ, ½ÇÇèÀýÂ÷¸¦ Á¤¸®ÇÑ ÀÚ·áÀÔ´Ï´Ù. ¾Æ¹«ÂÉ·Ï µµ¿òÀÌ µÇ±â¸¦ ¹Ù¶ó¸ç, ¸ðµÎ ÁÁÀº °á°ú ¹ÞÀ¸½Ã±æ ¹Ù¶ø´Ï´Ù. / 1. ¿ø¸® 2. ¹æ¹ý 3. ¿ëµµ 4. ½ÇÇèÀýÂ÷ / ¿©·¯°¡Áö ´Ü¹éµéÀ» Æ÷ÇÔÇÏ°í ÀÖ´Â ¼¼Æ÷ ¶Ç´Â Á¶Á÷ÀÇ ÃßÃâ¹°À» Àü±â ¿µµ¿ÇÏ¿© ±× ¼Ó¿¡ µé¾î ÀÖ´Â ´Ü¹éµéÀ» Å©±â º°·Î ±¸ºÐÇÑ ´ÙÀ½ ÈíÂøÁö¿¡ ¿Å°Ü ³õ°í Á¶»çÇÏ°íÀÚ ÇÏ´Â ´Ü¹éÀÇ Ç×ü¿Í °áÇÕ½ÃÄÑ ±× ´Ü¡¦ |
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³ë´ø ºí·ÔÆà (NORTHERN BLOTTING)ÀÇ ¿ø¸®¿Í ¿ëµµ, ¹æ¹ý, ½ÇÇèÀýÂ÷µéÀ» Á¤¸®ÇÑ ÀÚ·áÀÔ´Ï´Ù. °úÁ¦ ÀÛ¼ºÇϴµ¥ À¯¿ëÇÑ ³»¿ëÀÌ µÇ½Ã±æ ¹Ù¶ø´Ï´Ù. ´Ùµé ÁÁÀº Æò°¡ ¹ÞÀ¸½Ã±æ ¹Ù¶ø´Ï´Ù. / 1. ¿ø¸® 2. ¿ëµµ 3. ¹æ¹ý 4. ½ÇÇèÀýÂ÷ 1) Á¶Á÷ ¶Ç´Â ¹è¾ç¼¼Æ÷¿¡¼ÀÇ RNAºÐ¸® 2) Àü±â¿µµ¿À» À§ÇÑ RNA°Ëü ó¸® 3) ÇÑõ °ÖÀÇ Áغñ ¹× Àü±â¿µµ¿ 4) ÀüÀÌ 5) º¸ÇÕ°áÇÕ / ½á´ø ºÒ·ÔÆÃÀÌ DNA¸¦ ´Ù·ç´Â ÀÛ¾÷ÀΡ¦ |
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[ÀÌÈ¿©´ë »ý¸í°úÇнÇÇè1 ºÐ¹Ý1µî A£« ·¹Æ÷Æ®] Western Blotting / I. ½ÇÇè ¸ñÇ¥ II. Introduction 1. Western Blotting 2. SDS-PAGE 3. Antibody III. Methods 1. ½ÇÇè µµ±¸ (Materials) 2. ½ÇÇè ¹æ¹ý (Methods) ¨ç SDS-PAGE ¨è Gel Cassette Á¶¸³ ¹× Membrane Transfer ¨é Antibody Incubation ¨ê Detection IV. Discussion 1. Stacking gel°ú resolving gelÀÇ Â÷ÀÌ ¡¦ |
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SDS page gel electrophoresis ¹× western blotting ½ÇÇè ¸ñÀû ¹× ¿ø¸® º¸°í¼ / 1. ´Ü¹éÁú Àü±â¿µµ¿ 2. SDS-PAGE 1) Ư¼º 2) °úÁ¤ 3) ºÐÀÚ·® °áÁ¤ 4) Àû¿ë 3. Western blot 1) °úÁ¤ 2) Àû¿ë / 1. ´Ü¹éÁú Àü±â¿µµ¿ ´Ü¹éÁú Àü±â¿µµ¿Àº »ý¹°ÇÐÀû »ùÇÿ¡¼ ´Ü¹éÁúÀ» ºÐ¸®ÇÏ°í Á¤·®ÈÇÏ´Â Áß¿äÇÑ ±â¼ú·Î, ÁÖ·Î SDS-PAGE(Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophor¡¦ |
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