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¡¥lasmid DNA extraction 2) DNA sequencing 3. Methods 1) Chemicals & Apparatus 2) Procedure 4. Data & Results 1) Restriction enzyme information 2) Insert cut information 3) vector cut information 4) Ligated plasmid refernce 5) Sequencing result (raw) 6) Edited sequencing result 7) Editing process 5. Discussion / [½æ³×ÀÏÀ» È®ÀÎÇØ ÁÖ¼¼¿ä] |
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[ÀÇÇÐ,¾àÇÐ]»ý¹°ÇÐ - Bioinformatics & Sequencing º¸°í¼ / Bioinformatics & Sequencing 1. ½ÇÇè Á¦¸ñ Bioinformatics & Sequencing 2. ½ÇÇè¸ñÀû sequencing¿¡ ´ëÇØ ÀÌÇØÇÏ°í, sequencingÀ» ÅëÇØ ¾Ë ¼ö ÀÖ´Â DNA ¿°±â¼¿À» °¡Áö°í BLAST ºÐ¼®À» ÅëÇÏ¿© geneÀÇ ORF, homology, restriction enzyme site, motif, 2D structure µîÀ» ¾Ë¾Æº¸°í ÇÑ´Ù. 3. ½ÇÇè ¹æ¹ý ¨ç ½ÇÇè ¼ö¾÷ ½Ã°£¿¡¡¦ |
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¡¥±âº»ÀûÀÎ Á¤º¸¸¦ Á¦°øÇØÁÖ´Â Áß¿äÇÑ ½ÇÇèÀÌ´Ù. DNAÀÇ sequencingÀº 1977³â¿¡ À̸£·¯ °ÅÀÇ µ¿½Ã¿¡ °³¹ßµÈ µÎ °¡Áö ¹æ¹ý¿¡ ÀÇÇØ °¡´ÉÇÏ°Ô µÇ¾ú´Ù. ±× µÎ °¡Áö ¹æ¹ýÀº F. Sanger¿Í A. R. coulsonÀÌ °³¹ßÇÑ chain termination ¹æ¹ý°ú A. Maxam°ú W. GilbertÀÇ chemical degradation ¹æ¹ýÀÌ´Ù. ÇöÀç´Â ¿©·¯ °¡Áö ÀåÁ¡À¸·Î chain termination ¹æ¹ýÀ» ¼±È£ÇÏ°Ô µÇ¾ú´Ù. ±×·¡¼ ¿©±â¼´Â chain ¡¦ |
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11ÁÖÂ÷ sequencing BL21 transformation / I.Purpose II.Theory 1. Sanger sequencing 2. Competent cell(BL21(DE3)) III.Apparatus/reagent IV.Procedure 1. Cloning ½ÇÇè ÀÌÈÄ (preculture) 2. Preculture ´ÙÀ½³¯ 3. 11ÁÖÂ÷ ½ÇÇè¼ö¾÷ ´çÀÏ V.Data&Results VI.Discussion / I.Purpose BL21 ¼¼±ÕÀº ´ëÀå±ÕÀÇ ÇÑ °è¿·Î, ÀϹÝÀûÀ¸·Î ´Ü¹éÁú ¹ßÇö ½Ã½ºÅÛ¿¡¼ ³Î¸® »ç¿ëµÈ¡¦ |
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11ÁÖÂ÷ sequencing BL21 transformation / I.Purpose II.Theory 1. Sanger sequencing 2. Competent cell(BL21(DE3)) III.Apparatus/reagent IV.Procedure 1. Cloning ½ÇÇè ÀÌÈÄ (preculture) 2. Preculture ´ÙÀ½³¯ 3. 11ÁÖÂ÷ ½ÇÇè¼ö¾÷ ´çÀÏ V.Data&Results VI.Discussion / I.Purpose 11ÁÖÂ÷ÀÇ BL21 º¯È¯ ½ÇÇèÀº ÁÖ·Î ´Ü¹éÁú ¹ßÇö ¹× Á¤Á¦¸¦ À§ÇÑ Áß¿äÇÑ °úÁ¤À¸·Î, ´ë¡¦ |
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Next Generation Sequencing(NGS) ºÐ¼® ±â±âÀÇ ±â¼úÇõ½ÅÀü·« ºÐ¼®-Illumina ±â¼úÇõ½ÅÀü·« / ¥°. ¼·Ð 1. ¿°±â¼¿ ºÐ¼®¹ýÀÇ º¯È 2. ±â¾÷¼Ò°³ ¹× ¿¬±¸ ¸ñÀû ¥±. º»·Ð 1. IlluminaÀÇ ±â¼úÇõ½ÅÀü·« 2. °æÀï±â¾÷(1) - Thermo Fisher Scientific Inc. 3. °æÀï±â¾÷(2) - Roche 4. ½ÃÀå Àü¹Ý ºÐ¼® ¥². °á·Ð ¥³. Reference / ¥°. ¼·Ð Next Generation Sequencing(NGS) ±â¼úÀº »ý¡¦ |
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PlasmidDNA Purification , Sanger Sequencing ·¹Æ÷Æ® / 1. ½ÇÇè ¹è°æ 1-1 Bacterial transformation 1-2 Liquid LB culture ¹× Colony Inoculation 1-3 Plasmid purification 1-4 »ç¿ëÇÏ´Â BufferÀÇ Á¾·ù ¹× ¿ªÇÒ 1-5 Sanger Sequencing 1-6 Sequence È®ÀÎ ¹× Blast ÇÁ·Î±×·¥ 2. ½ÇÇè ¸ñÀû ¹× ¹æ¹ý - 2-1 ½ÇÇè ¸ñÀû 2-2 ½ÇÇè ¹æ¹ý 3. ½ÇÇè °á°ú 3-1 Chromas¸¦ ÅëÇÑ se¡¦ |
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[A0] ¼°´ë Çö´ë»ý¹°ÇнÇÇè2 4Â÷ Ç®·¹Æ÷Æ® - GFPuv¸¦ ÀÌ¿ëÇÑ Site-directed mutagenesisÀÇ ½Ç½À°ú DNA sequencing °á°úÀÇ ºÐ¼® ¿¬½À / 1. Abstract 2. Introduction 3. Materials & Method 4. Result 5. Discussion 6. Reference / 1. Abstract º» ½ÇÇèÀº GFPuv(Çü±¤ ´Ü¹éÁú)¸¦ ÀÌ¿ëÇÑ site-directed mutagenesis ±â¹ýÀ» Àû¿ëÇÏ¿© ƯÁ¤ À¯ÀüÀÚÀÇ º¯À̸¦ À¯µµÇÏ°í, ÀÌÀÇ ¼¿ ºÐ¡¦ |
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