|
[Å¥¾ËƼÀÚ±â¼Ò°³¼] Å¥¾ËƼÀÚ¼Ò¼,Å¥¾ËƼ(QRT)ÇÕ°ÝÀÚ±â¼Ò°³¼,Å¥¾ËƼ(QRT)ÀÚ¼Ò¼Ç׸ñ / Å¥¾ËƼ ÇÕ°Ý ÀÚ±â¼Ò°³¼ Å¥¾ËƼ ÀÚ±â¼Ò°³¼ ¿ì¼ö¿¹¹® 1. ÀÚ±â¼Ò°³¸¦ »ó¼¼È÷ ±âÀçÇÏ¿© Áֽñ⠹ٶø´Ï´Ù. 2. Áö¿øµ¿±â¿Í ÀÌÀ¯¸¦ ¼¼úÇÏ¿© ÁֽʽÿÀ. 3. ÀÌÁ¦±îÁö °¡Àå °ÇÑ ¼Ò¼Ó°¨À» ´À²¼´ø Á¶Á÷Àº ¹«¾ùÀ̸ç, ±× Á¶Á÷ÀÇ ¹ßÀüÀ» À§ÇØ Çå½ÅÀûÀ¸·Î ³ë·ÂÇÑ °Í °¡Àå ±â¾ï¿¡ ³²´Â °æÇèÀº ¹«¾ùÀԴϱî 4. ¡¦ |
|
|
|
|
|
1.RNA extraction 2.cDNA synthesis 3.Inhibitors for qRT-PCR I.Introduction »ý¸í.. / 1.RNA extraction 2.cDNA Synthesis 3.qRT-PCR III.Discussion / 1.RNA extraction 2.cDNA synthesis 3.Inhibitors for qRT-PCR I.Introduction »ý¸í°øÇÐ ºÐ¾ß¿¡¼ RNA ÃßÃâ, cDNA ÇÕ¼º, ±×¸®°í Á¤·®Àû ¿ªÀü»ç PCR(qRT-PCR)Àº À¯ÀüÀÚ ¹ßÇö ºÐ¼®¿¡ ÇʼöÀûÀÎ °úÁ¤ÀÌ´Ù. ÀÌ·¯ÇÑ °úÁ¤µé¡¦ |
|
|
|
|
|
[ºÐÀÚ»ý¹°ÇнÇÇè]Quantitative Real Time Polymerase Chain Reaction(qRT-PCR) / 1. ½ÇÇè¸ñÀû 2. ½ÇÇèÀÌ·Ð ¹× ¿ø¸® 3. ½ÇÇè±â±¸ ¹× ½Ã¾à 4. ½ÇÇè¹æ¹ý 5. ½ÇÇè°á°ú 6. ÅäÀÇ»çÇ× / 1. ½ÇÇè¸ñÀû Quantitative Real Time Polymerase Chain Reaction(qRT-PCR) ½ÇÇèÀÇ ¸ñÀûÀº ƯÁ¤ À¯ÀüÀÚÀÇ ¹ßÇö ¼öÁØÀ» Á¤·®ÀûÀ¸·Î ÃøÁ¤ÇÏ¿©, »ý¹°ÇÐÀû »ùÇÿ¡¼ À¯ÀüÀÚ ¹ßÇöÀÇ º¯È¸¦ ºÐ¼®ÇÏ´Â °ÍÀÌ´Ù¡¦ |
|
|
|
|
|
[½ÄÇ°ºÐ¼®ÇнÇÇè]DNA extraction , qRT-PCR / 1. ½ÇÇè ¸ñÀû 2. ½ÇÇè ÀÌ·Ð ¹× ¿ø¸® °¡. DNA extraction ³ª. qRT-PCR ´Ù. Amplification plot 3. ½ÇÇè ±â±¸ ¹× ½Ã¾à °¡. ½ÇÇè Àç·á 4. ½ÇÇè ¹æ¹ý °¡. Day 1 DNA Extraction ³ª. Day 2-3 qRT PCR ¹× °á°ú È®ÀÎ 5. ½ÇÇè °á°ú °¡. Day 1 (10/13) DNA Extraction ÈÄ spectrophotometer·Î Âï¾îº» °á°ú ³ª. Day 2-3 (10/14-15) qR¡¦ |
|
|
|
|
|
À¯Àü¹ß»ý»ý¹°ÇнÇÇè qRT-PCR / 1. ¼·Ð 2. Àç·á ¹× ¹æ¹ý 1) Àç·á 2) Reverse Transcription reaction ¹æ¹ý 3) qRT-PCR(Quantitative Real Time PCR) ¹æ¹ý 3. °á°ú 4. ³íÀÇ / 1. ¼·Ð À¯Àü¹ß»ý»ý¹°ÇÐ ½ÇÇèÀÇ ÇÑ ºÐ¾ßÀÎ qRT-PCR(Á¤·®Àû ¿ªÀü»ç ÁßÇÕÈ¿¼Ò ¿¬¼â ¹ÝÀÀ)Àº »ý¹°ÇÐÀû »ùÇà ³» Á¡ÁøÀûÀÎ À¯ÀüÀÚ ¹ßÇö ¼öÁØÀ» Á¤·®ÀûÀ¸·Î ºÐ¼®ÇÒ ¼ö ÀÖ´Â °·ÂÇÑ ¹æ¹ýÀ¸·Î ÀÚ¸®Àâ°í ÀÖ´Ù.¡¦ |
|
|
|
|
|
¼¼Æ÷»ý¹°ÇнÇÇè ¸¸Á¡(A£«) ½ÇÇè º¸°í¼(·¹Æ÷Æ®) 06. RNA ÃßÃâ, cDNA ÇÕ¼º ¹× qRT-PCR / ¼¼Æ÷»ý¹°ÇнÇÇè ¸¸Á¡(A+) ½ÇÇè º¸°í¼(·¹Æ÷Æ®) 06. RNA ÃßÃâ, cDNA ÇÕ¼º ¹× qRT-PCR ¸ñÂ÷ £±. ¼·Ð £². ½ÇÇè ¸ñÀû £³. ½ÇÇè ¹æ¹ý °¡.½ÇÇè Àç·á(µµ±¸) 1)»ùÇà 2)½Ã¾à 3)Àåºñ ³ª.½ÇÇè ¹æ¹ý £´. ½ÇÇè °á°ú £µ. ³íÀÇ (°íÂû) £¶. Âü°í¹®Çå £±. ¼·Ð RNA ÃßÃâ, cDNA ÇÕ¼º ¹× qRT-PCRÀº¡¦ |
|
|
|
|
|
ÀϹݻý¹°½ÇÇè ·¦ ¿µ¾î ·¹Æ÷Æ® 5. À¯ÀüÀÚ ¹ßÇö ¿¬±¸ (qRT-PCR) / 1. Introduction 2. Materials 3. Methods 4. Results 5. Discussion 6. References / 1. Introduction À¯ÀüÀÚ ¹ßÇö ¿¬±¸´Â »ý¹°ÇÐÀû °úÁ¤°ú ±â´ÉÀ» ÀÌÇØÇϱâ À§ÇÑ Áß¿äÇÑ ¹æ¹ý·Ð Áß ÇϳªÀÌ´Ù. À¯ÀüÀÚÀÇ ¹ßÇöÀº ¼¼Æ÷ ³»¿¡¼ À¯Àü Á¤º¸°¡ ´Ü¹éÁú µî ±â´ÉÀû ºÐÀÚ·Î º¯È¯µÇ´Â °úÁ¤À» ÀǹÌÇϸç, ÀÌ °úÁ¤Àº »ý¸íüÀÇ¡¦ |
|
|
|
|
|
¼¼Æ÷»ý¹°ÇнÇÇè ¸¸Á¡(A£«) ½ÇÇè º¸°í¼(·¹Æ÷Æ®) 06. RNA ÃßÃâ, cDNA ÇÕ¼º ¹× qRT-PCR / ¼¼Æ÷»ý¹°ÇнÇÇè ¸¸Á¡(A+) ½ÇÇè º¸°í¼(·¹Æ÷Æ®) 06. RNA ÃßÃâ, cDNA ÇÕ¼º ¹× qRT-PCR ¸ñÂ÷ £±. ¼·Ð £². ½ÇÇè ¸ñÀû £³. ½ÇÇè ¹æ¹ý °¡.½ÇÇè Àç·á(µµ±¸) 1)»ùÇà 2)½Ã¾à 3)Àåºñ ³ª.½ÇÇè ¹æ¹ý £´. ½ÇÇè °á°ú £µ. ³íÀÇ (°íÂû) £¶. Âü°í¹®Çå £±. ¼·Ð RNA ÃßÃâ, cDNA ÇÕ¼º ¹× qRT-PCRÀº¡¦ |
|
|
|
|