Purification °Ë»ö°á°ú

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[½ÇÇè] Purification of Thermostable DNA Polymerase

[½ÇÇè] Purification of Thermostable DNA Polymerase

º» ÀÚ·á´Â taq polymerase¸¦ Á¤Á¦Çϱâ À§ÇÑ ½ÇÇèÀÇ º¸°í¼­ÀÔ´Ï´Ù. PurificationofThermostableDNAPolymerase / 1. Title 2. Date 3. ½ÇÇèÀÚ 4. Theory 5. ½ÇÇè°úÁ¤ ¹× Áغñ¹° 6.Result 7. Discussion 8. Further study / 1st day (1)Seed Culture : 3ml LA media(LB + ampicillin)¿¡ single colony¸¦ Á¢Á¾ÇÑ ÈÄ 37¡É ¿¡¼­ overnight cultureÇÑ´Ù. 2nd day (1) 200ml LA media¡¦
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13ÁÖÂ÷ His-tagged protein purification

13ÁÖÂ÷ His-tagged protein purification

13ÁÖÂ÷ His-tagged protein purification / I.Purpose II.Theory 1. Affinity Chromatography (His-tag, Imidazole) 2. Ni-NTA agarose resin III.Apparatus/reagent IV.Procedure 1. His-tag Affinity Column Chromatography V.Data&Results VI.Discussion / I.Purpose His-tagged ´Ü¹éÁú Á¤Á¦ÀÇ ¸ñÀûÀº ÁַΠƯÁ¤ ´Ü¹éÁúÀ» È¿À²ÀûÀ¸·Î ºÐ¸®ÇÏ°í Á¤Á¦Çϱâ À§ÇÔÀÌ´Ù. Hi¡¦
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13ÁÖÂ÷ His-tagged protein purification

13ÁÖÂ÷ His-tagged protein purification

13ÁÖÂ÷ His-tagged protein purification / I.Purpose II.Theory 1. Affinity Chromatography (His-tag, Imidazole) 2. Ni-NTA agarose resin III.Apparatus/reagent IV.Procedure 1. His-tag Affinity Column Chromatography V.Data&Results VI.Discussion / I.Purpose His-tagged ´Ü¹éÁú Á¤Á¦ÀÇ ¸ñÀûÀº ƯÁ¤ ´Ü¹éÁúÀ» È¿À²ÀûÀ¸·Î ºÐ¸®ÇÏ°í Á¤Á¦ÇÏ¿© ¿¬±¸¿¡ È°¿ëÇÏ´Â °Í¡¦
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PCR purification ¿¹ºñ·¹Æ÷Æ®

PCR purification ¿¹ºñ·¹Æ÷Æ®

PCR purification ¿¹ºñ·¹Æ÷Æ® / 1. ¸ñÀû 2. Àç·á ¹× ¹æ¹ý 1) Àç·á 2) ¹æ¹ý / 1. ¸ñÀû PCR(Polymerase Chain Reaction) purificationÀÇ ¸ñÀûÀº PCR °úÁ¤¿¡¼­ »ý¼ºµÈ DNA ÇÁ·¡±×¸ÕÆ®¸¦ Á¤Á¦ÇÏ¿© ºÒÇÊ¿äÇÑ ¼ººÐÀ» Á¦°ÅÇÏ°í, ¼ø¼öÇÑ DNA »ùÇÃÀ» ¾ò´Â °ÍÀÌ´Ù. PCRÀº ƯÁ¤ DNA ¼­¿­À» ÁõÆøÇÏ´Â °­·ÂÇÑ ±â¼ú·Î, ¿¬±¸ ¹× Áø´Ü¿¡¼­ Æø³Ð°Ô »ç¿ëµÈ´Ù. ÇÏÁö¸¸ PCR °úÁ¤¿¡¼­ »ý¼ºµÈ ¹ÝÀÀ È¥¡¦
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Plasmid purification , DNA Á¤·® A£« report

Plasmid purification , DNA Á¤·® A£« report

Plasmid purification , DNA Á¤·® A£« report / 1.Introduction 2. Methods (mini-prep) 3. Result & Discussion 4. References / 1.Introduction Plasmid purification°ú DNA Á¤·® A+ report´Â »ý¸í°úÇÐ ¹× ºÐÀÚ »ý¹°ÇÐ ºÐ¾ß¿¡¼­ Áß¿äÇÑ ½ÇÇè °úÁ¤°ú °á°ú º¸°í¸¦ Æ÷ÇÔÇÑ´Ù. Çö󽺹̵å´Â ¿øÇÙ»ý¹°¿¡¼­ ÀÚÁÖ ¹ß°ßµÇ´Â ¿øÇüÀÇ ÀÌÁß³ª¼± DNA ºÐÀÚ·Î, ¼¼Æ÷ÀÇ À¯ÀüÀÚ Á¶ÀÛ ¹× Ŭ·Î´×¿¡¡¦
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PlasmidDNA Purification , Sanger Sequencing ·¹Æ÷Æ®

PlasmidDNA Purification , Sanger Sequencing ·¹Æ÷Æ®

PlasmidDNA Purification , Sanger Sequencing ·¹Æ÷Æ® / 1. ½ÇÇè ¹è°æ 1-1 Bacterial transformation 1-2 Liquid LB culture ¹× Colony Inoculation 1-3 Plasmid purification 1-4 »ç¿ëÇÏ´Â BufferÀÇ Á¾·ù ¹× ¿ªÇÒ 1-5 Sanger Sequencing 1-6 Sequence È®ÀÎ ¹× Blast ÇÁ·Î±×·¥ 2. ½ÇÇè ¸ñÀû ¹× ¹æ¹ý - 2-1 ½ÇÇè ¸ñÀû 2-2 ½ÇÇè ¹æ¹ý 3. ½ÇÇè °á°ú 3-1 Chromas¸¦ ÅëÇÑ se¡¦
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Protein purification °á°úº¸°í¼­

Protein purification °á°úº¸°í¼­

Protein purification °á°úº¸°í¼­ / 1. ½ÇÇèÁ¦¸ñ 2. ½ÇÇè¸ñÀû 3. ½ÇÇè¹æ¹ý 4. ½ÇÇè°á°ú 5. °íÂû 6. Âü°í¹®Çå / 1. ½ÇÇèÁ¦¸ñ ½ÇÇè Á¦¸ñÀº `´Ü¹éÁú Á¤Á¦ ¹× Ư¼º ºÐ¼®`ÀÌ´Ù. ÀÌ ½ÇÇèÀÇ ¸ñÀûÀº ƯÁ¤ ´Ü¹éÁúÀ» ÀÚ¿¬¿¡¼­ ÃßÃâÇÏ°í, À̸¦ Á¤Á¦ÇÏ¿© ±× Ư¼ºÀ» ºÐ¼®ÇÏ´Â °úÁ¤ÀÌ´Ù. ´Ü¹éÁúÀº »ý¸íü ³»¿¡¼­ ¼öÇàÇÏ´Â ´Ù¾çÇÑ ±â´É ¶§¹®¿¡ Áß¿äÇÑ »ýüºÐÀÚ·Î, ¿¬±¸ÀÇ ¹°ÁúÀû ±âÃÊ°¡ µÈ´Ù. ´Ü¡¦
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Protein purification ¿¹ºñº¸°í¼­

Protein purification ¿¹ºñº¸°í¼­

Protein purification ¿¹ºñº¸°í¼­ / 1. ½ÇÇè¸ñÀû 2. ½ÇÇèÀÌ·Ð 3. ½ÇÇè¹æ¹ý 4. ½Ã¾àÁ¶»ç 5. Âü°í¹®Çå / 1. ½ÇÇè¸ñÀû ´Ü¹éÁú Á¤Á¦ÀÇ ¸ñÀûÀº ƯÁ¤ ´Ü¹éÁúÀ» ºÐ¸®ÇÏ°í ¼ø¼öÇÏ°Ô ¾ò´Â °ÍÀ¸·Î, »ý¹°ÇÐÀû ¿¬±¸ ¹× ÀÀ¿ë¿¡¼­ ÇÙ½ÉÀûÀÎ ´Ü°èÀÌ´Ù. ¸¹Àº »ý¹°ÇÐÀû °úÁ¤¿¡¼­ ´Ü¹éÁúÀº Áß¿äÇÑ ¿ªÇÒÀ» Çϸç, À̵éÀÇ ±â´É°ú ±¸Á¶¸¦ ÀÌÇØÇÏ´Â °ÍÀº »ý¸í °úÇÐ ºÐ¾ß¿¡¼­ ÇʼöÀûÀÌ´Ù. ±×·¯³ª ¼¼Æ÷ ³»¿¡¡¦
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purification of plasmid from bactetial cells ¿¹ºñ

purification of plasmid from bactetial cells ¿¹ºñ

purification of plasmid from bactetial cells ¿¹ºñ / 1. ½ÇÇè¸ñÀû 2. ½ÇÇèÀÌ·Ð 3. ½ÇÇè¹æ¹ý 4. ½Ã¾àÁ¶»ç 5. Âü°í¹®Çå / 1. ½ÇÇè¸ñÀû ½ÇÇè¸ñÀûÀº ´ëÀå±Õ µîÀÇ ¼¼±Õ¿¡¼­ Çö󽺹̵带 ÃßÃâÇÏ°í Á¤Á¦ÇÏ´Â °úÁ¤À» ÅëÇØ °í¼øµµÀÇ Çö󽺹̵带 È®º¸ÇÏ´Â °ÍÀÌ´Ù. Çö󽺹̵å´Â ¼¼±ÕÀÇ À¯ÀüÀÚ Á¶ÀÛ, ´Ü¹éÁú ¹ßÇö, ÇÕ¼º »ý¹°ÇÐ µî ´Ù¾çÇÑ ºÐ¾ß¿¡¼­ Áß¿äÇÑ ¿ªÇÒÀ» Çϸç, À̸¦ ¿¬±¸ÇÏ°í È°¡¦
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purification method by recrystallization °á°ú

purification method by recrystallization °á°ú

purification method by recrystallization °á°ú / 1. ½ÇÇèÁ¦¸ñ 2. ½ÇÇè¸ñÀû 3. ½ÇÇè¹æ¹ý 4. ½ÇÇè°á°ú 5. °íÂû 6. Âü°í¹®Çå / 1. ½ÇÇèÁ¦¸ñ ½ÇÇè Á¦¸ñÀº `Àç°áÁ¤È­¸¦ ÅëÇÑ ¹°Áú Á¤Á¦ ¹æ¹ý`ÀÌ´Ù. Àç°áÁ¤È­´Â È­ÇÐ ½ÇÇè¿¡¼­ ³Î¸® »ç¿ëµÇ´Â ±â¼ú·Î, °íü ¹°ÁúÀ» ¼ø¼öÇÑ °áÁ¤ ÇüÅ·ΠÁ¤Á¦ÇÏ´Â °úÁ¤ÀÌ´Ù. ÀÌ ¹æ¹ýÀº ÀϹÝÀûÀ¸·Î È¥ÇÕ¹°¿¡¼­ ºÒ¼ø¹°À» Á¦°ÅÇÏ°í, ¹°ÁúÀÇ ¼øµµ¸¦ ³ôÀ̱â À§¡¦
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