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1. Title Adherent Cell Lysis for Protein Extraction ¼¼Æ÷ »ý¹°ÇÐ ¹× »ýÈÇÐ ¿¬±¸¿¡¼ ´Ü¹éÁú ÃßÃâÀº Áß.. / 1. Title 2. Date 3. Name 4. Purpose 5. Materials 6. Methods 7. Results 8. Discussions 9. Project 10. Reference / 1. Title Adherent Cell Lysis for Protein Extraction ¼¼Æ÷ »ý¹°ÇÐ ¹× »ýÈÇÐ ¿¬±¸¿¡¼ ´Ü¹éÁú ÃßÃâÀº Áß¿äÇÑ °úÁ¤ÀÌ´Ù. ƯÈ÷, ºÎÂø ¼¼Æ÷¡¦ |
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Cell Transfection and Cell lysis ·¹Æ÷Æ® / 1. Background Cell Transfection 1-1 Cell transfection 1-2 HEK-293T cell 1-3 Epitope tagging 1-4 Transfection reagent Cell Lysis 1-a Cell lysis 1-b Lysis buffer 2. Purpose and Method Cell Transfection 2-1 Purpose 2-2 Method Cell Lysis 2-a Purpose 2-b Method 3. Result - 3-1 Cell picture and Cell lysis ¡¦ |
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/ 1. Title Adherent Cell Lysis for Protein ExtractionÀº ¼¼Æ÷ »ý¹°ÇÐ ¹× »ýÈÇÐ ºÐ¾ß¿¡¼ Áß¿äÇÑ ÁÖÁ¦ÀÌ´Ù. ¸¹Àº ¿¬±¸¿¡¼ ´Ü¹éÁúÀÇ ±â´É, ±¸Á¶ ¹× »óÈ£ÀÛ¿ëÀ» ÀÌÇØÇϱâ À§ÇØ ¼¼Æ÷ ³» ´Ü¹éÁúÀ» ÃßÃâÇÏ´Â °úÁ¤ÀÌ ÇʼöÀûÀÌ´Ù. ¼¼Æ÷´Â ´Ù¾çÇÑ ¹°Áú·Î ±¸¼ºµÇ¾î ÀÖÀ¸¸ç, ƯÁ¤ ´Ü¹éÁúÀ» ¿¬±¸Çϱâ À§Çؼ´Â ¼¼Æ÷¸·À» ¿øÈ°ÇÏ°Ô Æı«ÇÏ°í ³»ºÎÀÇ ´Ü¹éÁúÀ» È¿°úÀûÀ¸·Î ÃßÃâÇØ¾ß ÇÑ´Ù. ÀÌ´Â ¡¦ |
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Cell Transfection and Cell lysis ·¹Æ÷Æ® / 1. Background Cell Transfection 1-1 Cell transfection 1-2 HEK-293T cell 1-3 Epitope tagging 1-4 Transfection reagent Cell Lysis 1-a Cell lysis 1-b Lysis buffer 2. Purpose and Method Cell Transfection 2-1 Purpose 2-2 Method Cell Lysis 2-a Purpose 2-b Method 3. Result - 3-1 Cell picture and Cell lysis ¡¦ |
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SDS-PAGE , cell lysis, buffer°ü·Ã ·¹Æ÷Æ® (A£«ÀÚ·á) / 1. SDS-PAGE 1) ¿ø¸® 2) Á¦Á¶¹æ¹ý 3) ÇÊ¿äÇÑ ½Ã¾à buffer 4) ½ÇÇè½Ã ÁÖÀÇ»çÇ× 2. Cell Lysis / 1. SDS-PAGE ¥â-¸Þ¸£ÄºÅ信ź¿Ã ¶Ç´Â µðƼ¿À½º·Î·¹ÀÌÆ®)¸¦ »ç¿ëÇÏ¿© ´Ü¹éÁúÀÇ ÀÌÈ²È °áÇÕÀ» ²÷´Â °úÁ¤ÀÌ Æ÷ÇԵȴÙ. À̸¦ ÅëÇØ ´Ü¹éÁúÀº ¼±Çü »óÅ·Πº¯ÇØ Àü±â¿µµ¿ ½Ã ´õÇÏ°Ô À̵¿ÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ, »ùÇÿ¡ ºÒÇÊ¿äÇÑ ¹°ÁúÀ̳ª ¡¦ |
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Plasmid DNA preparation¿¹ºñ·¹Æ÷Æ®(Alkaline lysis miniprep,Boiling miniprep,Lithium miniprep) / 1. Subject 2. Introduction / 1. Subject Plasmid DNA preparationÀº À¯ÀüÀÚ Å¬·Î´× ¹× ºÐÀÚ »ý¹°ÇÐ ½ÇÇè¿¡¼ Áß¿äÇÑ ±â¼úÀÌ´Ù. ÀÌ °úÁ¤Àº ¼¼±Õ ³»¿¡¼ Çö󽺹̵带 ºÐ¸®ÇÏ°í Á¤Á¦ÇÏ´Â ¹æ¹ýÀ¸·Î, ½ÇÇèÀÚµéÀÌ ¿øÇÏ´Â À¯Àü Á¤º¸¸¦ ½±°Ô ´Ù·ê ¼ö ÀÖµµ·Ï ÇØÁØ´Ù. Çö󽺹̵å´Â ÀÚ°¡ ¡¦ |
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Cell lysis , Protein assay / 1. ¼·Ð 2. Àç·á ¹× ¹æ¹ý 3. °á°ú 4. °íÂû 5. Âü°í¹®Çå / 1. ¼·Ð Cell lysis and protein assays are fundamental techniques in molecular biology, biochemistry, and cell biology that play a crucial role in understanding cellular functions and biochemical pathways. The objective of cell lysis is to disrupt the cellular membrane,¡¦ |
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1. ½ÇÇè ¸ñÀû ¹× ¿ø¸® Lysis Buffer Á¦Á¶ ¹× Cell Lysate ¼öÁýÀÇ ¸ñÀûÀº ¼¼Æ÷ ³» À¯ÀüÀÚ, ´Ü¹éÁú, ¹× ±âŸ »ýü ºÐÀÚÀÇ .. / 1. ½ÇÇè ¸ñÀû ¹× ¿ø¸® 2. ½Ã·á, ½Ã¾à ¹× ±â±¸ 3. ½ÇÇè ¹æ¹ý 4. ½ÇÇè °á°ú 5. °íÂû 6. Âü°í ¹®Çå / 1. ½ÇÇè ¸ñÀû ¹× ¿ø¸® Lysis Buffer Á¦Á¶ ¹× Cell Lysate ¼öÁýÀÇ ¸ñÀûÀº ¼¼Æ÷ ³» À¯ÀüÀÚ, ´Ü¹éÁú, ¹× ±âŸ »ýü ºÐÀÚÀÇ ºÐ¼®À» À§ÇÑ ±âÃÊ ÀڷḦ Á¦°øÇÏ¡¦ |
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1. Tumor lysis syndrome Tumor lysis syndromeÀº Á¾¾ç Ä¡·áÀÇ °á°ú·Î ¹ß»ýÇÏ´Â ´ë»ç ÀÌ»óÀ¸·Î, ÁÖ·Î ¾Ç¼º Á¾¾ç.. / 1. Tumor lysis syndrome 2. Tumor lysis syndromeTreatment 3. Spinal Cord Compression 4. SPINAL CORD COMPRESSION TREATMENT / 1. Tumor lysis syndrome Tumor lysis syndromeÀº Á¾¾ç Ä¡·áÀÇ °á°ú·Î ¹ß»ýÇÏ´Â ´ë»ç ÀÌ»óÀ¸·Î, ÁÖ·Î ¾Ç¼º Á¾¾çÀÇ ±Þ¼ÓÇÑ ±«»ç ¡¦ |
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