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  • Cell Transfection and Cell lysis ·¹Æ÷Æ®   (1 ÆäÀÌÁö)
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Cell Transfection and Cell lysis ·¹Æ÷Æ®

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ÆÄÀÏ : Cell Transfection and Cell lysis ·¹Æ÷Æ®.hwp   [Size : 55 Kbyte ]
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1. Background

Cell Transfection
1-1 Cell transfection

1-2 HEK-293T cell

1-3 Epitope tagging

1-4 Transfection reagent

Cell Lysis
1-a Cell lysis
1-b Lysis buffer
2. Purpose and Method
Cell Transfection
2-1 Purpose
2-2 Method
Cell Lysis
2-a Purpose
2-b Method
3. Result -
3-1 Cell picture and Cell lysis centrifuge picture
3-2 Conclusion and Discussion
4. Reference
º»¹®/³»¿ë
1. Background

Cell transfection°ú cell lysis´Â ºÐÀÚ »ý¹°ÇÐ ¹× ¼¼Æ÷ »ý¹°ÇÐ ¿¬±¸¿¡¼­ Áß¿äÇÑ ±â¼úÀÌ´Ù. Cell transfectionÀº ¿ÜºÎ À¯Àü ¹°ÁúÀ» ¼¼Æ÷ ³»ºÎ·Î Àü´ÞÇÏ´Â °úÁ¤À» ¶æÇϸç, À̸¦ ÅëÇØ Æ¯Á¤ À¯ÀüÀÚÀÇ ¹ßÇöÀ» Á¶ÀýÇϰųª ´Ü¹éÁúÀÇ ±â´ÉÀ» ¿¬±¸ÇÒ ¼ö ÀÖ´Ù. ÀϹÝÀûÀ¸·Î DNA, RNA ¶Ç´Â ´Ü¹éÁú°ú °°Àº À¯ÀüÀÚ Àç·á°¡ ¼¼Æ÷¿¡ ÅõÀԵǸç, ÀÌ °úÁ¤¿¡¼­ ¼¼Æ÷¸·À» ³Ñ´Â ´Ù¾çÇÑ ¹æ¹ýÀÌ »ç¿ëµÈ´Ù. È­ÇÐÀû ¹æ¹ý, ¹°¸®Àû ¹æ¹ý, ¹ÙÀÌ·¯½º ¸Å°³¹ý µîÀÌ ÀÖÀ¸¸ç, °¢ ¹æ¹ýÀº »óÀÌÇÑ È¿À²°ú ¾ÈÀü¼º, ¼¼Æ÷ À¯Çü¿¡ µû¶ó Àå´ÜÁ¡ÀÌ ÀÖ´Ù. ¿¹¸¦ µé¾î, ¸®Æ÷Á»À» ÀÌ¿ëÇÏ´Â È­ÇÐÀû ¹æ¹ýÀº ºñ±³Àû °£´ÜÇÏ°í ¸¹Àº ¼¼Æ÷¿¡¼­ »ç¿ëµÉ ¼ö ÀÖÀ¸¸ç, Àü±âõ°ø¹ýÀº ³ôÀº È¿À²À» ÀÚ¶ûÇÏÁö¸¸ ¼¼Æ÷¿¡ ´ëÇÑ ½ºÆ®·¹½º°¡ Å©´Ù. ¹ÙÀÌ·¯½º ¸Å°³¹ýÀº ³ôÀº ÀüÀÌ È¿À²¼º ¶§¹®¿¡ À¯ÀüÀÚ Àü´Þ¿¡ ³Î¸® »ç¿ëµÇÁö¸¸, ¹ÙÀÌ·¯½ºÀÇ ¾ÈÀü¼º ¹®Á¦µµ °í·ÁÇØ¾ß ÇÑ´Ù. Transfection ±â¼úÀº ÁÖ·Î À¯ÀüÀÚ ±â´É ¿¬±¸, ´Ü¹éÁú ¹ßÇö, ´ë»ç °æ·Î Çؼ®, Ä¡·áÁ¦ °³¹ß µî ´Ù¾çÇÑ ºÐ¾ß¿¡¼­ È°¿ëµÈ´Ù. ¿¹¸¦ µé¾î, ƯÁ¤ ´Ü¹éÁúÀÇ ¹ßÇöÀ» Áõ°¡½ÃÅ°°Å³ª ¾ïÁ¦ÇÔÀ¸·Î½á ÇØ´ç ´Ü¹éÁúÀÇ »ý¸®ÇÐÀû ¿ªÇÒÀ» ¿¬±¸ÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ, ƯÁ¤ À¯ÀüÀÚÀÇ ¹ßÇöÀ»¡¦(»ý·«)


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ID : hajo******
Regist : 2024-09-29
Update : 2024-09-29
FileNo : 24998826

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Cell   Transfection   lysis  


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