|
½ÄÇ°°¡°ø¿¡¼ ´Ü¹éÁúÀÇ º¯Çü¸ñÀû°ú º¯Çü¹æ¹ý °úÁ¤ µî¿¡ ´ëÇØ Á¶»çÇÑ ÀÚ·áÀÔ´Ï´Ù. Modificationoffoodproteins / 1) ´Ü¹éÁú º¯Çü (Protein modification) 2) º¯Çü ¹æ¹ý 3) ½ÄÇ°°¡°ø¿¡¼ ´Ü¹éÁúÀÇ º¯Çü ¸ñÀû 1.ÈÇÐÀû º¯Çü (Chemical modification) 2. È¿¼ÒÀû º¯Çü (enzymatic modification) 3. ¹°¸®Àû º¯Çü (Physical modification) / (3) ½Ã½ºÅ×ÀÎ(cysteine)°ú ½Ã½ºÆ¾(cystine)ÀÇ »ê¡¦ |
|
|
|
|
|
¡¥n¡± - alpha-helix rich prion protein (PrPC) ¡æa beta-structure-rich insoluble confor mer (PrPSc) prion `ÀüÀÚÇö¹Ì°æÀ¸·Î º» prion µ¢¾î¸®` prion prp-sc (prion protein-scrap |
|
|
|
|
|
¹ÙÀÌ·¯½ºÇÐ ½ÇÇè °á°úº¸°í¼·Î Cell harvest & Protein sampling ½ÇÇè¿¡ ´ëÇÑ °á°ú º¸°í¼ÀÔ´Ï´Ù. Cell harvest & Protein sampling ½ÇÇèÀºpoliovirus¿¡ °¨¿°µÈ BGM cellÀ» ¿ëÇؽÃŲ ÈÄ, ´Ü¹éÁúÀ» ÃßÃâÇÏ°í Á¤·®ÇÏ´Â ½ÇÇèÀÔ´Ï´Ù. ´Ü¹éÁúÀ» ÃßÃâÇÏ´Â ÀÌÀ¯´Â SDS-PAGE¸¦ Çؼ Å©±â¿¡ µû¶ó ´Ü¹éÁúÀ» ºÐ¸® / 1. Title 2. Objective 3. Method 1) Cell harvest 2) Protein sampling ¡¦ |
|
|
|
|
|
ÀÚ¿¬°úÇкΠ¼ö¼®ÀÇ ½ÇÇè·¹Æ÷Æ® ½Ã¸®Áî¿¡ ¿À½Å °ÍÀ» ȯ¿µÇÕ´Ï´Ù. ¿©·¯°¡Áö ¼Ò½º¸¦ Ãѵ¿¿øÇÏ¿© ÇØ´ç ½ÇÇè¿¡ ´ëÇÑ ÀÌ·Ð ¹× ¹è°æÁö½ÄÀ» Á¶»çÇÏ°í, ½ÇÇè ¹æ¹ý°ú °á°ú, ÅäÀÇ¿¡ ÇØ´çÇÏ´Â ºÎºÐÀº ÃÖ´ëÇÑ Àü¹®Áö½ÄÀ» »ì¸®¸é¼µµ ¿©·¯ºÐÀÌ ÀÌÇØÇϱâ ÁÁµµ·Ï »ó¼¼ÇÏ°í °¡µ¶¼º ÀÖ°Ô ÀÛ¼ºÇß´Ù°í ÀÚºÎÇÕ´Ï´Ù. ¿©·¯ºÐÀÇ ½ÇÇè°ú º¸°í¼ ÀÛ¼º¿¡ Å« µµ¿òÀÌ µÇ±æ ¹Ù¶ø´Ï´Ù. / 1. Purpose 2. Theory 1) pET ve¡¦ |
|
|
|
|
|
Lowry protein assay (´Ü¹éÁú Á¤·® ºÐ¼®) ½ÇÇè ¿¹ºñ·¹Æ÷Æ® [A+] / ¿¹ºñ·¹Æ÷Æ® ½Ç Çè Á¦ ¸ñ : Lowry protein assay (´Ü¹éÁú Á¤·® ºÐ¼®) Á¶ : ÇÐ ¹ø : ÀÌ ¸§ : 1. ½ÇÇè ¸ñÀû UV¹ý, Lowry¹ý, BCA¹ý°ú Bradford¹ý µîÀÇ ´Ù¾çÇÑ ´Ü¹éÁúÀÇ Á¤·®À» ºÐ¼®ÇÏ´Â ¹æ¹ýµé¿¡ ´ëÇØ ÀÌÇØÇÏ°í, Lowry method¸¦ ÀÌ¿ëÇÏ¿© ´Ù¾çÇÑ ³óµµÀÇ ´Ü¹éÁú ½Ã·á¸¦ Á¤·®Çغ»´Ù. ¶ÇÇÑ UV/Vis spectrophotometerÀÇ À̿롦 |
|
|
|
|
|
Lowry protein assay (´Ü¹éÁú Á¤·® ºÐ¼®) ½ÇÇè °á°ú·¹Æ÷Æ® [A+] / °á°ú¸®Æ÷Æ® ½ÇÇèÁ¦¸ñ : Lowry protein assay (´Ü¹éÁú Á¤·® ºÐ¼®) Á¶ : ÇÐ ¹ø : ÀÌ ¸§ : 1. Abstract À̹ø ½ÇÇèÀº Lowry ¹ýÀ» ÀÌ¿ëÇÏ¿© ´Ù¾çÇÑ ³óµµ¸¦ °¡Áø ´Ü¹éÁú ½Ã·á ¿ë¾×À» Á¤·®ÇÏ´Â ½ÇÇèÀÌ´Ù. ¸ÕÀú Èñ¼®½ÃŲ ´Ù¾çÇÑ ³óµµÀÇ BSA¿ë¾× 5°³¿Í ¹ÌÁö ³óµµÀÇ BSA¿ë¾× 1°³¿¡ Lowry Reagent¿Í Folin Reagent ¸¦ ¼ø¼´ë·Î ³Ö¡¦ |
|
|
|
|
|
[ÀÚ¿¬°úÇÐ][°øÇлý¹°ÇÐ] ºê·¡µåÆ÷µå ´Ü¹éÁú Á¤·®¹ý[The Bradford Method for Protein Quantitation] / °øÇÐ »ý¹°ÇÐ ¹× ½ÇÇè ·¹Æ÷Æ® 1. Á¦¸ñ The Bradford Method for Protein Quantitation 2. Áغñ¹° Bradford ½Ã¾à Protein Standard Solution Spectrophotometer¿Í Quartz cuvette, Pipet Milk, Áõ·ù¼ö ep tube BSA Protein(standard) 3. ½ÇÇè °úÁ¤ Milk¸¦ Áõ·ù¼ö¸¦ ÀÌ¿ëÇÏ¿© ,¡¦ |
|
|
|
|
|
[°øÇÐ]»ýÈÇÐ - Methods of protein estimation / ¡¶»ýÈÇС· Methods of protein estimation - ¸ñ Â÷ - ¥°. ¼·Ð ¥±. º»·Ð 1. Lowry method - ¿ø¸® - ½Ã¾à ¹× ±âÀç - ¹æ¹ý - ´Ù¸¥ method¿ÍÀÇ Â÷ÀÌÁ¡ - Âü°í) ºä·¿ ½ÃÇè (biuret test) 2. Bredford method - ½Ã¾à ¹× Àç·á - ¹æ¹ý - ´Ù¸¥ method¿ÍÀÇ Â÷ÀÌÁ¡ 3. BCA method - ¹æ¹ý - ´Ù¸¥ method¿ÍÀÇ Â÷ÀÌÁ¡ Âü°í¹®Çå ¥°. ¼·Ð »ý¸í¡¦ |
|
|
|
|
|
¡¥ kinetic energy barrier ¶§¹®¿¡ proteinÀº ÀÌ·± misfoldedµÈ »óÅ·ΠÁ¸Àç -³·Àº ¿Âµµ¿¡ÀÇ ³ëÃâÀº cold denaturation : reverse enthalpic & entropic effect·Î ÀϾ 2) pH -formulation bufferÀÇ pH´Â ÈÇÐÀûÀ¸·Î amino aò Àܱ⠹ٲٴ protein solubility, folding, ¹ÝÀÀ¼Óµµ¿¡ ½É°¢ÇÑ Ãæ°ÝÀ» ÁÙ ¼ö ÀÖ´Ù. 3) Ionic Strength À̿°µµ - counterionÀÇ ³óµµ´Â ºÐÀÚ¿¡¼ Á¤Àü±â¡¦ |
|
·¹Æ÷Æ® >
±âŸ  | 
133p age   | 
3,000 ¿ø
|
|
|
|
|
|
proteasome - ÁøÇÙ»ý¹°¿¡ º¸ÆíÀûÀ¸·Î Á¸ÀçÇÏ´Â °Å´ë ÇÁ·ÎÅ×¾ÆÁ¦(protease, ´Ü¹éÁúºÐÇØÈ¿¼Ò)ÀÇ 1Á¾. 1980³â ... / proteasome - ÁøÇÙ»ý¹°¿¡ º¸ÆíÀûÀ¸·Î Á¸ÀçÇÏ´Â °Å´ë ÇÁ·ÎÅ×¾ÆÁ¦(protease, ´Ü¹éÁúºÐÇØÈ¿¼Ò)ÀÇ 1Á¾. 1980³â ÃÊ¿¡ ¼ÒÀÇ ³úÇϼöü¿¡¼ óÀ½ ¹ß°ßµÇ¾ú´Ù. È¿¸ð¸¦ ºñ·ÔÇÏ¿© ½Ä¹°°ú Æ÷À¯·ù¿¡ À̸£±â±îÁö °ÅÀÇ ¸ðµç ÁøÇÙ¼¼Æ÷ÀÇ ÇÙ°ú ¼¼Æ÷Áú¿¡ Á¸ÀçÇÏ°í ÀÖ´Ù. 700 kDa ÀÌ¡¦ |
|
|
|
|