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´Ü¹éÁú Á¤·®°ú proteasome activity assay / ¿ä¾à ¼·Ð ½ÇÇè Àç·á ¹× ¹æ¹ý °á°ú °íÂû Âü°í¹®Çå FileSize : 96K / ¿ä¾à ¼·Ð ½ÇÇè Àç·á ¹× ¹æ¹ý °á°ú °íÂû Âü°í¹®Çå / ´Ü¹éÁú Á¤·®À» ÇÑ ¸ñÀûÀº °¢ ´Ü¹éÁúÀÇ ³óµµ°¡ ´Ù¸£±â ¶§¹®¿¡ °°Àº °ªÀ» ¾ò±â À§ÇØ °¢ ±â°üÀÇ ¹ß´Þ¿¡ µû¶ó Á¶Á÷À» ¾ò¾î³»´Â °ÍÀ» ¸ñÀûÀ¸·Î ÇÑ´Ù. ±×·¸°Ô Çؼ ¾òÀº ´Ü¹éÁúÀ»ÆļâÇÑ ÈÄ Lowry method¿¡ ÀÇÇØ Á¤·®°ª¡¦ |
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¹ÙÀÌ·¯½ºÇÐ ½ÇÇè °á°úº¸°í¼·Î Plaque assay ½ÇÇè¿¡ ´ëÇÑ °á°ú º¸°í¼ÀÔ´Ï´Ù. Plaque assay ½ÇÇèÀº ¹ÚÅ׸®¿À ÆÄÁöÀÎ phi-x-174¸¦ Plaque assayÇÏ¿© ÇöóÅ©ÀÇ Çü¼º À¯¹«·Î ¹ÙÀÌ·¯½ºÀÇ À¯¹«¸¦ È®ÀÎÇÏ´Â ½ÇÇèÀÔ´Ï´Ù. º»¹®³»¿ëÀº ½ÇÇè ¸ñÇ¥, ¿ø¸®, ½ÇÇè ¹æ¹ý¿¡ ´ëÇÑ ÀÚ¼¼ÇÑ ¼³¸í, °á°ú »çÁø°ú ´À³¤Á¡ µîÀ» Æ÷ÇÔÇÏ°í ÀÖ½À´Ï´Ù. / 1. Title 2. Objective 3. Principle 4. Method 5. Res¡¦ |
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È¿¼ÒÀÇ È°¼º ½ÇÇè Enzyme activity assay ¿¹ºñ·¹Æ÷Æ® [A+] / ¿¹ºñ·¹Æ÷Æ® ½ÇÇèÁ¦¸ñ : Enzyme activity assay Á¶ : ÇÐ ¹ø : ÀÌ ¸§ : 1. ½ÇÇè ¸ñÀû - »ý¼º¹°ÀÇ ³óµµ¸¦ UV spectrophotometer¸¦ ÀÌ¿ëÇÏ¿© ÃøÁ¤ÇÏ°í ÃøÁ¤°ªÀ» ¹ÙÅÁÀ¸·Î ½Ã°£´ç Èí±¤µµ ±×·¡ÇÁ¸¦ ±×·Áº»´Ù. ±×¸®°í È¿¼ÒÀÇ È°¼º°ú ºñÈ°¼º¿¡ ´ëÇØ ¾Ë¾Æº»´Ù. 2. ¹ÙÅÁ ÀÌ·Ð (1) È¿¼Ò (Enzyme) ¨ç È¿¼ÒÀÇ Á¤ÀÇ - È¿¼Ò´Â ¼¼Æ÷ ³»¡¦ |
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È¿¼ÒÀÇ È°¼º ½ÇÇè Enzyme activity assay °á°ú·¹Æ÷Æ® [A+] / °á°ú·¹Æ÷Æ® ½Ç Çè Á¦ ¸ñ : Enzyme activity assay Á¶ : ÇÐ ¹ø : ÀÌ ¸§ : 1. Abstract À̹ø ½ÇÇèÀº 50mM Tris-HCl buffer ¿Í 50 mM p-NPBÀ» ³Ö¾î 0.5 mM pNPB ¿ë¾×À» ¸¸µé°í, ¿©±â¿¡ ½ÇÇ豺 Bottle¿¡ calB È¿¼Ò¸¦ 20 ¥ìL ³ÖÀº µÚ ½Ã°£ÀÌ Áö³²¿¡ µû¶óUV spectrophotometer¸¦ ÀÌ¿ëÇØ ¿ë¾×ÀÇ Èí±¤µµ¸¦ ÃøÁ¤ÇÏ´Â ½ÇÇèÀ̾ú´Ù. À̷Ρ¦ |
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[ÀÚ¿¬°úÇÐ]»ý¸íÈÇнÇÇè - RT-PCR, Bradford assay, SDS page, Genomic DNA prep / »ý¸íÈÇÐ ½ÇÇè Report (RT-PCR, Bradford assay, SDS page, Genomic DNA prep) 1.Introduction RT-PCR DNA ¿¡¼´Â exon°ú intronÀÌ ÀÖÀ¸¹Ç·Î DNA¸¦ template·Î ÇÏ´Â °æ¿ì¿¡´Â exon°ú intronÀÌ ¸ðµÎ ÁõÆøµÉ ¼ö ÀÖ´Ù. ÇÏÁö¸¸ intronÀº exon¿¡ ºñÇؼ ¸Å¿ì Å©±â ¶§¹®¿¡ exon Áß¿¡ ¸î °³¸¦ ¡¦ |
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Lowry protein assay (´Ü¹éÁú Á¤·® ºÐ¼®) ½ÇÇè ¿¹ºñ·¹Æ÷Æ® [A+] / ¿¹ºñ·¹Æ÷Æ® ½Ç Çè Á¦ ¸ñ : Lowry protein assay (´Ü¹éÁú Á¤·® ºÐ¼®) Á¶ : ÇÐ ¹ø : ÀÌ ¸§ : 1. ½ÇÇè ¸ñÀû UV¹ý, Lowry¹ý, BCA¹ý°ú Bradford¹ý µîÀÇ ´Ù¾çÇÑ ´Ü¹éÁúÀÇ Á¤·®À» ºÐ¼®ÇÏ´Â ¹æ¹ýµé¿¡ ´ëÇØ ÀÌÇØÇÏ°í, Lowry method¸¦ ÀÌ¿ëÇÏ¿© ´Ù¾çÇÑ ³óµµÀÇ ´Ü¹éÁú ½Ã·á¸¦ Á¤·®Çغ»´Ù. ¶ÇÇÑ UV/Vis spectrophotometerÀÇ À̿롦 |
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Lowry protein assay (´Ü¹éÁú Á¤·® ºÐ¼®) ½ÇÇè °á°ú·¹Æ÷Æ® [A+] / °á°ú¸®Æ÷Æ® ½ÇÇèÁ¦¸ñ : Lowry protein assay (´Ü¹éÁú Á¤·® ºÐ¼®) Á¶ : ÇÐ ¹ø : ÀÌ ¸§ : 1. Abstract À̹ø ½ÇÇèÀº Lowry ¹ýÀ» ÀÌ¿ëÇÏ¿© ´Ù¾çÇÑ ³óµµ¸¦ °¡Áø ´Ü¹éÁú ½Ã·á ¿ë¾×À» Á¤·®ÇÏ´Â ½ÇÇèÀÌ´Ù. ¸ÕÀú Èñ¼®½ÃŲ ´Ù¾çÇÑ ³óµµÀÇ BSA¿ë¾× 5°³¿Í ¹ÌÁö ³óµµÀÇ BSA¿ë¾× 1°³¿¡ Lowry Reagent¿Í Folin Reagent ¸¦ ¼ø¼´ë·Î ³Ö¡¦ |
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[ÀÚ¿¬°úÇÐ]»ý¸íÈÇнÇÇè - RT-PCR, Bradford assay, SDS page, Genomic DNA prep / »ý¸íÈÇÐ ½ÇÇè Report (RT-PCR, Bradford assay, SDS page, Genomic DNA prep) 1.Introduction RT-PCR DNA ¿¡¼´Â exon°ú intronÀÌ ÀÖÀ¸¹Ç·Î DNA¸¦ template·Î ÇÏ´Â °æ¿ì¿¡´Â exon°ú intronÀÌ ¸ðµÎ ÁõÆøµÉ ¼ö ÀÖ´Ù. ÇÏÁö¸¸ intronÀº exon¿¡ ºñÇؼ ¸Å¿ì Å©±â ¶§¹®¿¡ exon Áß¿¡ ¸î °³¸¦ ¡¦ |
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Protein determination and Proteasome activity assay / proteasome - ÁøÇÙ»ý¹°¿¡ º¸ÆíÀûÀ¸·Î Á¸ÀçÇÏ´Â °Å´ë ÇÁ·ÎÅ×¾ÆÁ¦(protease, ´Ü¹éÁúºÐÇØÈ¿¼Ò)ÀÇ 1Á¾. 1980³â ... / proteasome - ÁøÇÙ»ý¹°¿¡ º¸ÆíÀûÀ¸·Î Á¸ÀçÇÏ´Â °Å´ë ÇÁ·ÎÅ×¾ÆÁ¦(protease, ´Ü¹éÁúºÐÇØÈ¿¼Ò)ÀÇ 1Á¾. 1980³â ÃÊ¿¡ ¼ÒÀÇ ³úÇϼöü¿¡¼ óÀ½ ¹ß°ßµÇ¾ú´Ù. È¿¸ð¸¦ ºñ·ÔÇÏ¿© ½Ä¹°°ú Æ÷À¯·ù¿¡ À̸£±â±îÁö °ÅÀÇ ¡¦ |
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¡¥ Á×´Â ´Ù¸é Ç×¾Ï È¿°ú°¡ ÀÖ´Ù´Â ¶æ 2. MTT assay (reduce) MTT(yellow)-------------------->MTT - formazan(purple) ¡è Succinic dehydrogenase ¿ø¸® TCA cycle¿¡¼ cycleÀÌ µ¹ ¶§ succinate¿¡¼ fumarate·Î °¡´Â °úÁ¤¿¡¼ succinic dehydrogenase °¡ ÇÊ¿äÇÏ°í ÀÌ cycle¿¡ °ü¿©ÇÑ´Ù. succinate ¡æ Fumarate·Î °¡´Â °úÁ¤¿¡¼ MTT¸¦ Åõ¿©ÇÏ´Â µ¥ ¿©±â¿¡¼ MTT¿Í succinate dehydrog¡¦ |
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