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11ÁÖÂ÷ sequencing BL21 transformation / I.Purpose II.Theory 1. Sanger sequencing 2. Competent cell(BL21(DE3)) III.Apparatus/reagent IV.Procedure 1. Cloning ½ÇÇè ÀÌÈÄ (preculture) 2. Preculture ´ÙÀ½³¯ 3. 11ÁÖÂ÷ ½ÇÇè¼ö¾÷ ´çÀÏ V.Data&Results VI.Discussion / I.Purpose 11ÁÖÂ÷ÀÇ BL21 º¯È¯ ½ÇÇèÀº ÁÖ·Î ´Ü¹éÁú ¹ßÇö ¹× Á¤Á¦¸¦ À§ÇÑ Áß¿äÇÑ °úÁ¤À¸·Î, ´ë¡¦ |
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