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1. Title : Cell harvest & Protein sampling
2. Objective : �겕湲곗뿉 �뵲�씪 �떒諛깆쭏�쓣 遺꾨━�븯�뒗 SDS-PAGE瑜� �븯湲곗쐞�빐�꽌 poliovirus�뿉 媛먯뿼�맂 BGM cell�쓣 �슜�빐�떆�궓 �썑, �떒諛깆쭏�쓣 異붿텧�븯怨� �젙�웾�븳�떎.
3. Method
1) Cell harvest
�몺 諛곗��瑜� e-tube�뿉 �닔嫄고븳�떎.
�몼 PBS濡� washing �븯怨� 1ml PBS瑜� 異붽���븳�떎.
�몾 Scraper濡� 湲곸뼱 紐⑥븘 e-tube�뿉 �떞�뒗�떎.
�몿 5,000rpm/5min centrifugation
�뫀 �긽�벑�븸�쓣 suction�빐�꽌 pellet�쓣 痍⑦븳�떎.
2) Protein sampling
�몺 Pellet�뿉 lysis buffer瑜� �꽔�뒗�떎.
�몼 Voltexing�븯�뿬 pellet�쓣 �궡吏� ����뼱以� �썑 deep freezer (-80�꼦)�뿉 15遺꾧컙 �꽔�뼱�몦�떎.
�몾 37�꼦 water bath�뿉 �궡吏� �끃�씤 �썑 centrifugation (13,000rpm, 15min, 4�꼦)
�몿 �긽�벑�븸�쓣 �깉濡쒖슫 e-tube�뿉 �삷湲대떎.
�뫀 O.D媛믪쓣 痢≪젙�븳�떎.
(以묐왂)
5. Discussion
�씠踰� �떎�뿕�쑝濡쒕뒗 BGM cell�쓣 sub cultre�븯怨� seeding �븳 �썑 virus瑜� 媛먯뿼�떆�궓 BGM cell�쓣 �닔嫄고븯�뿬�꽌 Cell harvest �썑 Protein sampling�쓣 �넻�빐 �떒諛깆쭏�쓣 異붿텧�븯怨� �젙�웾�뻽�떎.
癒쇱�� Cell harvest濡� 諛곗��瑜� e-tube�뿉 �닔嫄고븳�떎. PBS 1.5ml�뵫 �꽔�뼱 washing �븯怨� 1ml PBS瑜� 異붽���븳�떎. 諛곗���쓽 �떒諛깆쭏�쓣 �젣嫄고븯湲� �쐞�빐 washing�쓣 瑗쇨세�엳 �빐以��떎.
(以묐왂)
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