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1. �떎�뿕 紐⑹쟻
�몺plasmid�쓽 �솕�븰�쟻 遺꾨━踰뺤쓣 �씡�엺�떎.
�몼plasmid�쓽 �젙�쓽��� �듅吏뺤쓣 �븣�븘蹂몃떎.
�몾洹좎쓽 pure culture�뿉 ����빐 �븣�븘蹂몃떎.
�몿�쟾湲곗쁺�룞, �썝�떖遺꾨━ �벑�쓽 �궗�슜踰뺤쓣 �씡�엺�떎.
2.�떎�뿕 �썝由�
�몺Bacterial DNA�뒗 plasmid DNA蹂대떎 �썾�뵮 �겕�떎.
�몼Bacterial DNA�뒗 �뿬�윭援곕뜲媛� �옒�젮吏� linear DNA �씤 諛섎㈃�뿉 plasmid�뒗 covalently covalently closed circular DNA�씠�떎. �뵲�씪�꽌 ephidium bromide 泥섎━�떆 covalently closed circular DNA�뒗 linear DNA蹂대떎 �썾�뵮 �쟻寃� ephidium bromide媛� 遺�李⑸맖�쑝濡쒖뜥 CsCl density ultracentrifugation�븯寃뚮릺硫�, �긽����쟻�쑝濡� �넂��� 諛��룄 援щ같�뿉�꽌 band瑜� �삎�꽦�븳�떎.
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Plasmid Purification.hwp
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