PCR 怨� DNA replication�섏빟蹂닿굔�덊룷��

PCR 怨� DNA replication

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자료설명

PCR 怨� DNA replication�� 먮즺�낅땲��.
遺꾩옄�앸Ъ�숇━�ы듃

본문/내용

�덉슂�썬��

1) PCR

- 以묓빀�⑥냼 �곗뇙諛섏쓳(polymerase chain reaction, PCR)�� 뱀젙 DNA 遺��꾨� �뱀씠�곸쑝濡� 諛섎났 �⑹꽦�섏뿬 �쒗뿕愿��댁뿉�� 먰븯�� DNA 遺꾩옄瑜� 利앺룺�쒗궎�� 諛⑸쾿�쇰줈 �꾩＜ �곸� �묒쓽 DNA瑜� �댁슜�섏뿬 留롮� �묒쓽 DNA �⑹꽦�� 媛��ν븯��.
PCR�� DNA遺꾩옄�� 뱀젙 遺��꾨� �좏깮�곸쑝濡� 利앺룺�쒗궓��. �대뼡 DNA遺꾩옄�� 대뼡 遺��꾨씪�� 좏깮�� 덈떎. Border sequence�ㅼ� PCR�� 섑뻾�섍린 �꾪빐�� 뚮젮�� 덉뼱�� 쒕떎. �먰븳 �� 媛쒖쓽 吏㏃� oligonucleotide媛� �댁쨷�섏꽑�� 媛� 媛��κ낵 �쇱꽦�붾릺�댁빞留� �쒕떎. DNA�⑹꽦諛섏쓳�� 꾪븳 primer濡쒖뜥 ��븷�� 섎뒗 �대뱾 oligonucleotide�ㅼ� 利앺룺�� 遺��꾩쓽 踰붿쐞瑜� �뺥빐以��.
�� PCR 怨쇱젙
1) Pre-PCR status : PCR �� ㅼ삩�� buffer condition�먯꽌�� DNA�� 덉젙�곸씤 B form double helix援ъ“瑜� �대（怨� �덈떎. templates�쇨퀬 遺�瑜닿린�� 쒕떎.

2) Denaturation : 怨듭쑀 寃고빀�쇰줈 �대（�댁졇 �덈떎. PCR�먯꽌 base pairs媛꾩쓽 hydrogen bond瑜� �딄린 �꾪빐 �ъ슜�섎뒗 energy�� 蹂댄넻 90oC源뚯� �댁쓣 媛�� 硫� single strnad濡� ��댁쭊��.

3) Annealing : PCR�� 理쒖쥌�곸씤 sensitivity�� specificity瑜� 寃곗젙�섎뒗 �쒖씪 以묒슂 �� 怨쇱젙.

4) Extension : Taq polymerase媛� primer�� 3�셞nd�� free OH湲곕� �쒖옉�먯쑝濡� �댁꽌 single strand template�� 곷낫�곸씤 dNTP瑜� 媛��몄�� 遺숈뿬 �섍�硫� chain extension�� 쒗뻾�쒕떎.

2) DNA replication
- DNA 遺꾩옄�� 곷낫�곸씤 �쇨린�띿뿉 �섑븳 �댁쨷��(�앸왂)

참고문헌

�댁젙洹�, �ㅼ꽍以�, 源�醫낅� ��, 諛붿씠�ㅼ씤�щℓ�깆뒪, �쒕튆 誘몃뵒��, 2001
Snyder and Champness, `Molecular genetics of Bacteria` 1997
DNA Microarray�� 理쒖떊 PCR 湲곕쾿 �섏쑄�� (2000)
http://www.immunoblot.com
http://www.komabiotech.com
http://www.bmskorea.co.kr
http://www.chinju.ac.kr/~hak31/down/DNA Replication.hwp
http://kimwootae.com.ne.kr/mb/dnareplication.htm
http://www.dongeui.ac.kr/%7Ecblee/lec05/bio05.html
http://www.knou.ac.kr/~bhkim/study52.htm

장바구니

PCR 怨� DNA replication

1) PCR

2) Denaturation : 怨듭쑀 寃고빀�쇰줈 �대（�댁졇 �덈떎. PCR�먯꽌 base pairs媛꾩쓽 hydrogen bond瑜� �딄린 �꾪빐 �ъ슜�섎뒗 energy�� 蹂댄넻 90oC源뚯� �댁쓣 媛��� 硫� single strnad濡� ���댁쭊��.

3) Annealing : PCR�� 理쒖쥌�곸씤 sensitivity�� specificity瑜� 寃곗젙�섎뒗 �쒖씪 以묒슂 �� 怨쇱젙.

4) Extension : Taq polymerase媛� primer�� 3�셞nd�� free OH湲곕� �쒖옉�먯쑝濡� �댁꽌 single strand template�� �곷낫�곸씤 dNTP瑜� 媛��몄��� 遺숈뿬 �섍�硫� chain extension�� �쒗뻾�쒕떎.

2) Denaturation : 怨듭쑀 寃고빀�쇰줈 �대（�댁졇 �덈떎. PCR�먯꽌 base pairs媛꾩쓽 hydrogen bond瑜� �딄린 �꾪빐 �ъ슜�섎뒗 energy�� 蹂댄넻 90oC源뚯� �댁쓣 媛�� 硫� single strnad濡� ��댁쭊��.

4) Extension : Taq polymerase媛� primer�� 3�셞nd�� free OH湲곕� �쒖옉�먯쑝濡� �댁꽌 single strand template�� 곷낫�곸씤 dNTP瑜� 媛��몄�� 遺숈뿬 �섍�硫� chain extension�� 쒗뻾�쒕떎.