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1.�떎�뿕�궡�슜
-�떎�뿕紐⑹쟻: sandwich ELISA瑜� �씠�슜�븯�뿬 媛� �삁泥��쓽 IgM�쓣 �젙�웾,�젙�꽦遺꾩꽍 �븳�떎.
-�떎�뿕以�鍮꾨Ъ: Capture Ab(Goat 慣-Dog IgM), Blocking Reagent(3%BSA/PBS),
Detection Ab(Goat 慣-Dog IgM-HRP), PBS-T, TMB, stop solution,
ELISA reader, pipette, tube, plate, 嫄곗쫰, Dog serum.
-�떎�뿕怨쇱젙(�떎�젣 �뻽�뜕 �떎�뿕�씠 �븘�땶 �빐�빞�뻽�뜕 �떎�뿕怨쇱젙)
i) A~H源뚯���엳�뒗 plate�쓽 媛곴컖�쓽 well�뿉 Capture Ab(Goat 慣-Dog IgM)瑜� �꽔�뼱以��떎.
�넂sandwich ELISA瑜� �쐞�빐 plate諛붾떏�뿉 Ab瑜� 肄뷀똿�븯湲곗쐞�빐�꽌
ii) �꽔�뿀�뜕 Capture Ab瑜� 踰꾨━怨� PBS-T(200關l)瑜� �씠�슜�빐 3�쉶 �뵽�뼱以��떎.
�넂�꽔怨�,�쓷�뱾怨�,�꽭怨좊�� 3�쉶諛섎났. washing�씠 �뜑 �옒�릺�룄濡� �빐二쇰뒗 媛쒕㈃�솢�꽦�젣�씤 Tween20
�쓣 �꽔�뼱以� PBS-T�뒗 遺덉닚臾쇱쓣 �젣嫄고빐以��떎.
iii) 100關l�쓽 Blocking Reagent(3%BSA/PBS)瑜� �꽔�뼱以섏꽌 Capture Ab�쇅�쓽 臾쇱쭏�씠 plate
諛붾떏�뿉 �떖�씪遺숈�� 紐삵븯�룄濡� 肄뷀똿�빐以��떎. 洹몃━怨� 1�떆媛� 湲곕떎由고썑 PBS-T濡� 3�쉶�뵽�뼱以��떎.
iv) 100關l�쓽 �냽�룄瑜� �븣�닔�뾾�뒗 Dog serum�쓣 A�뿉 �꽔怨�, 200關l�쓽 100諛� �씗�꽍�맂 Dog serum �쓣 B�뿉 �꽔�뒗�떎. C�뿉 100關l�쓽 PBS��� B�슜�븸�쓽 100關l瑜� �꽔怨� �꽎����떎�쓬 D�뿉 100關l�쓣 �삷 湲댄썑 D�뿉 100關l�쓽 PBS瑜� �꽔怨� �꽎����떎�쓬......G源뚯�� �븯�떎蹂대㈃ 媛곴컖�쓽 Plate�뒗 100關l�쓽 �냽�룄媛� �떎瑜� �슜�븸�씠 �뱾�뼱�엳寃뚮맂�떎.
(A:�냽�룄紐⑤쫫 B:10ng/ml, C:5ng/ml, D:2.5ng/ml, E:1.25ng/ml, F:0���(�깮�왂)
2.ELISA reader or Microplate reader or Spectrophotometer
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硫댁뿭�븰 �떎�뿕 �젅�룷�듃.hwp
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