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�솚寃쎌뿉 �뵲瑜� Amylase �솢�꽦�룄
1. �떎�뿕紐⑹쟻
Amylase媛� �뼱�뼡 �삩�룄���, �뼱�뼡 pH�뿉�꽌 �솢�꽦�룄媛� 媛��옣 �넂���吏� �븞�떎.
2. �썝由�
1) Enzyme
�깮紐낆껜 �궡遺��쓽 �솕�븰 諛섏쓳�쓣 留ㅺ컻�븯�뒗 Protein 珥됰ℓ�씠�떎. Enzyme��� Temperament��� 寃고빀�븯�뿬 Enzymesubstrate
complex(�슚�냼-湲곗쭏 蹂듯빀泥�)瑜� �삎�꽦�븿�쑝濡쒖뜥 諛섏쓳�쓽 �솢�꽦�솕 �뿉�꼫吏�瑜� �궙異붾뒗 珥됰ℓ �뿭�븷�쓣 �븳�떎. 媛� 醫낅쪟�쓽 eb�쓽 �씠由꾩��
���媛� `-�뿉�씠�뒪(-ase)`濡� �걹�궃�떎. Enzyme��� Temperament�뿉 ����븳 �듅�씠�꽦�쓣 媛�吏�怨� �엳�쑝硫�, �씪諛섏쟻�쑝濡� �긽�삩�뿉�꽌 泥댁삩 �젙
�룄�쓽 �삩�룄��� 以묒꽦 pH�뿉�꽌 �옒 �옉�룞�븳�떎. �븯吏�留� �듅�씠�븳 �깮紐낆껜�쓽 Enzyme�뱾��� 洹뱁븳 議곌굔(�삁: �꽠�뵪 72�룄, pH 2�쓽 媛뺥븳 �궛
�꽦 �긽�깭 �벑)�뿉�꽌�룄 �옉�룞�븷 �닔 �엳�떎. Enzyme��� 珥됰ℓ�븯�뒗 諛섏쓳�쓽 醫낅쪟��� 諛섏쓳�븯�뒗 Temperament�쓽 醫낅쪟�뿉 �뵲�씪 EC
number(�슚�냼 踰덊샇)濡� 遺꾨쪟�맂�떎.
2) Amylase
Starch�씠�굹 Glycogen �벑 Glucose瑜� 援ъ꽦�떦(礪뗦닇楹�)�쑝濡� �븯�뒗 �떎�떦瑜섎�� 媛��닔遺꾪빐�븯�뒗 �슚�냼�쓽 珥앹묶. �옉�슜�뼇�떇�뿉 �뵲�씪 慣 -�븘諛��씪�븘�젣 棺-Amylase Glucoamylase�쓽 3醫낆쑝濡� 遺꾨쪟�맂�떎. 移� �냽�뿉�뒗 �빟 0.4g�쓽 Amylase媛� �븿�쑀�릺�뼱 �엳�쑝硫�, 移� �씠�굹 �씠�옄�븸 �냽�쓽 Amylase�뒗 Starch�쓣 媛��닔遺꾪빐�븯�뿬 Maltose瑜� �깮�꽦�븯誘�濡� �냼�솕�옉�슜�뿉 遺덇��寃고븯�떎. Amylase�뒗 怨좊벑�룞 臾쇰퓧留� �븘�땲�씪 怨좊벑�떇臾� 諛� 怨고뙜�씠 �꽭洹� �벑 �옄�뿰怨꾩뿉 �꼸由� 遺꾪룷�븳�떎.
3) Starch
怨좊벑�떇臾쇱쓽 �꽭�룷�뿉 �엳�뒗 Starch�쓽 寃곗젙�씠�굹 洹멸쾬�쓣 異붿텧�븳 寃껋쓣 �씪諛섏쟻�쑝濡� Starch�씪怨� 遺덈━硫�, 遺꾩옄�떇(C6H12O6)n �쓽
Carbohydrate�뿉 �떎�닔�쓽 慣-Glucose 遺꾩옄媛� Gly���(�깮�왂)
4) Iodine solution
3. �떆猷� 諛� 湲곌뎄
4. �떎�뿕諛⑸쾿
(1) �삩�룄�쓽 �쁺�뼢
1) 3媛쒖쓽 E-tube�뿉 1-3源뚯�� labelling �븳�떎.
2) 媛� E-tube�뿉 瑜� 0.01g �뵫 �꽔�뒗�떎.
3) 媛� tube�뿉 Starch solution�쓣 800關l�뵫 �꽔�뒗�떎.
4) 1踰� tube�뒗 0�꼦 (ice), 2踰� tube�뒗 37�꼦 (water), 3踰� tube�뒗 70�꼦 (water)�뿉 �꽔�뒗�떎.
5) 諛섏쓳�떆媛� 5遺�.
6) 5遺� �썑, 1-3踰� tube�뿉 Iodine potassium iodine solution�쓣 200ul�뵫 �꽔�뒗�떎.
7) 諛쒖깋 諛섏쓳�쓣 愿�李고븳�떎.
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