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�깮臾쇳븰�떎�뿕 - DNA 異붿텧, PCR�쓽 �썝由� 諛� �떎�뿕
`�떎�뿕 諛⑸쾿`
-DNA 異붿텧
1. Solution 1
2. Solution 2
3. Solution 3
4. Washing
5. Elution
-PCR
1. PCR tube�뿉 dNTPs buffer 4 關l��� reaction buffer 5 關l瑜� �꽔�뒗�떎.
2. Template DNA 1 關l瑜� �꽔怨� forward, reverse primer瑜� 媛곴컖 0.5 關l �뵫 �꽔�뒗�떎.
3. DNA polymerase 1 關l瑜� �꽔�뒗�떎.
4. 利앸쪟�닔 37 關l瑜� �꽔怨� �옒 pipetting �빐 以��떎.
5. PCR machine�쓽 well�뿉 tube瑜� �꽔怨� 諛섏쓳�떆�궓�떎.
`�떎�뿕 �옱猷�`
-PCR
DNA polymerase, Template DNA, primers, dNTPs mixture, Reaction buffer, PCR tube, 利앸쪟�닔, pipette tip, pipette
`miniprep�쓽 �썝由�`
Plasmid DNA extraction
Plasmid瑜� 遺꾨━�빐�궡�뒗 諛⑸쾿��� 異붿텧�웾�뿉 �뵲�씪 miniprep, midiprep, maxiprep �씠 �엳�떎.
湲곕낯�쟻�씤 �썝由щ뒗 �꽭洹좎쓽 Cell wall�쓣 Alkali lyssis method瑜� �넻�빐 源⑤���닔怨� DNA瑜� 遺꾨━ �븳 �썑 �떎�떆 Chromosomal DNA��� plasmid DNA 以� plasmid留뚯쓣 遺꾨━�븯�뒗 寃껋씠�떎.
�떎�뿕�뿉 �궗�슜�븳 �슜�븸�쓽 �뿭�븷�쓣 �븣�븘蹂대룄濡� �븯�옄.
Solution I
Solution II
Solution III
50mM Glucose
25mM Tris-Cl (pH 8.0)
10mM EDTA ���(�깮�왂)
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