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1. Á¦¸ñ
The Bradford Method for Protein Quantitation
2. Áغñ¹°
Bradford ½Ã¾à
Protein Standard Solution
Spectrophotometer¿Í Quartz cuvette, Pipet
Milk, Áõ·ù¼ö
ep tube
BSA Protein(standard)
3. ½ÇÇè °úÁ¤
Milk¸¦ Áõ·ù¼ö¸¦ ÀÌ¿ëÇÏ¿© , , ¿ø¾×ÀÇ ºñÀ²·Î dilutionÇÑ´Ù.
Bradford ½Ã¾àÀ» 1§¢¾¿ ep tube¿¡ 7°³ ³Ö´Â´Ù.
Standard SoulutionÀ» 0§¡, 2§¡, 4§¡, 6§¡¾¿ tube¿¡ ³Ö´Â´Ù.
diluted milk¸¦ 5§¡¾¿ ep tube¿¡ ³Ö´Â´Ù.
°ÅÇ°ÀÌ ³ªÁö ¾Êµµ·Ï õõÈ÷ µÚÁýÀ¸¸ç ¼¯´Â´Ù.
°¢°¢ ½Ã·á¸¦ 1.5§¢ cuvettes¿¡ 1§¢¾¿ ³Ö´Â´Ù.
7.595§¬ ÆÄÀå¿¡¼ Standard SolutionÀÇ Èí±¤µµ¸¦ ÃøÁ¤ÇÑ ÈÄ ±â·ÏÇÑ´Ù.
³ª¸ÓÁö ½Ã·áÀÇ Èí±¤µµ¸¦ ÃøÁ¤ÇÑ ÈÄ ±â·ÏÇÑ´Ù.
4. ¼·Ð
Bradford Method´Â »ê¼º ¿ë¾×¿¡¼ ºÒ±×½º¸§ÇÑ °¥»öÀ» ¶ç´Â Coomassie Brilliant Blue G-250 (CBBG) ¿°·áÀÇ Èí±¤µµ º¯È¸¦ ÃøÁ¤ÇÑ´Ù. CBBG°¡ free dyeÇüÅ·ΠÀÖÀ» ¶§ÀÇ ÃÖ´ë Èí±¤Àº 465§¬(»¡°)ÀÌÁö¸¸, ´Ü¹éÁúÀÇ ¾Æ¹Ì³ë»ê°ú °áÇÕÇÏ¿© anio¡¦(»ý·«)
5. °á°ú
1) ºñÀ²ÀÇ diluted milk : 0.166 ¡ë 166
2) ºñÀ²ÀÇ diluted milk : 1.79 ¡ë 1790
3) undiluted milk : 6.986 ¡ë 6986
6. ÅäÀÇ ¹× °ÇÀÇ»çÇ×
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