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  • [»ý¹°ÇÐ º¸°í¼­] DNA Isolation, PCR, Àü±â¿µµ¿ ½ÇÇè   (1 ÆäÀÌÁö)
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[»ý¹°ÇÐ º¸°í¼­] DNA Isolation, PCR, Àü±â¿µµ¿ ½ÇÇè

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ÆÄÀÏ : [»ý¹°ÇÐ º¸°í¼­] DNA Isolation, PCR, Àü±â¿µµ¿ ½ÇÇè.hwp   [Size : 4 Mbyte ]
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-Date
-Title

¨çDNA Isolation

¨èPCR(polymerase chain reaction)

¨éÀü±â¿µµ¿(electrophoresis)

-Object

¨ç¹ÚÅ׸®¾Æ·ÎºÎÅÍ DNA¸¦ ºÐ¸®ÇØ ³½´Ù.

¨èƯÁ¤ DNA ºÎÀ§¸¦ ¹Ýº¹ ÇÕ¼ºÇÏ¿© ¿øÇÏ´Â DNA ºÐÀÚ¸¦ ÁõÆø ½ÃŲ´Ù.

¨éÀü±â¿µµ¿Àº DNA¸¦ Å©±â¿¡ µû¶ó¼­ ºÐ¸®ÇÏ´Â ¹æ¹ýÀÌ´Ù.

-Materials&
Methods

¨çCell lysis sol, proteinase K, RNase, PPT sol, e-tube, DNA binding sol, spin colmn, column washing sol, water, ¿ø½ÉºÐ¸®±â

1. Cell lysis sol 300§¡ (inverting)

2. proteinase K 10§¡ (inverting) 56¡É¿¡¼­ 15ºÐ

3. RNase A 3§¡ 37¡É¿¡¼­ 15ºÐ

4. PPT sol 100§¡¡æ Vortexing¡æ In ice¿¡¼­ 5ºÐ

5. 10ºÐ µ¿¾È 1...

º»¹®/³»¿ë
-Date

-Title
¨çDNA Isolation
¨èPCR(polymerase chain reaction)
¨éÀü±â¿µµ¿(electrophoresis)
-Object
¨ç¹ÚÅ׸®¾Æ·ÎºÎÅÍ DNA¸¦ ºÐ¸®ÇØ ³½´Ù.
¨èƯÁ¤ DNA ºÎÀ§¸¦ ¹Ýº¹ ÇÕ¼ºÇÏ¿© ¿øÇÏ´Â DNA ºÐÀÚ¸¦ ÁõÆø ½ÃŲ´Ù.
¨éÀü±â¿µµ¿Àº DNA¸¦ Å©±â¿¡ µû¶ó¼­ ºÐ¸®ÇÏ´Â ¹æ¹ýÀÌ´Ù.
-Materials&
Methods
¨çCell lysis sol, proteinase K, RNase, PPT sol, e-tube, DNA binding sol, spin colmn, column washing sol, water, ¿ø½ÉºÐ¸®±â

1. Cell lysis sol 300§¡ (inverting)

2. proteinase K 10§¡ (inverting) 56¡É¿¡¼­ 15ºÐ
3. RNase A 3§¡ 37¡É¿¡¼­ 15ºÐ

4. PPT sol 100§¡¡æ Vortexing¡æ In ice¿¡¼­ 5ºÐ

5. 10ºÐ µ¿¾È 1300rpm centrifuge
6. »óµî¾× 390§¡¡æ e-tube¡æ DNA binding sol 600§¡
7. 650§¡ in Spin column¡æ 1ºÐ µ¿¾Ècentrifuge
8. Column washing sol 600§¡¡æ 1ºÐ µ¿¾Ècentrifuge
9. Column washing sol 500§¡¡æ 1ºÐ µ¿¾Ècentrifuge
10. spin columnÀ» »õ e tube¿¡ ¿Å±â°í water 100§¡¸¦ ³Ö°í 5ºÐ Á¤Áö ÈÄ 1ºÐ °£ centrifuge.

¨èDNA polymerase, Template DNA, primers, dNTPs, mixture, reaction buffer, PCR tube, Áõ·ù¼ö, pipette ¡¦(»ý·«)


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ID : leew*****
Regist : 2012-05-31
Update : 2017-03-23
FileNo : 11043091

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»ý¹°ÇÐ   º¸°í¼­   DNA   Isolation   PCR   Àü±â¿µµ¿   ½ÇÇè  


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